Analysis Note

ControlBrain tissue

Application

Research CategoryNeuroscience

Immunohistochemistry:
1:1000-1:2000 dilution of a previous lot was used in immunohistochemistry (see suggested protocol).

ELISA:
1:1000-1:2000 dilution of a previous lot was used in ELISA.

Bioassay:
Does not inhibit BDNF biological activity.

Optimal working dilutions must be determined by end user.

Research Sub CategoryNeurochemistry & NeurotrophinsNeuroinflammation & Pain

This Anti-Brain Derived Neurotrophic Factor Antibody is validated for use in ELISA, IH, WB for the detection of Brain Derived Neurotrophic Factor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Brain Derived Neurotrophic Factor (BDNF) is a secreted protein that promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders. BDNF exists as monomers or homodimers and binds to TRKB/NTRK2 receptors.

Immunogen

A 10 amino acid peptide corresponding to the amino-terminal of mouse Brain Derived Neurotrophic Factor (BDNF) coupled to ovalbumin.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Rabbit polyclonal antiserum in buffer containing no preservatives. Lyophilized. Reconstitute with 50 µL of sterile distilled water.

Unpurified

Quality

Evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected BDNF on 10 µg of mouse brain lysates.

Specificity

Brain Derived Neurotrophic Factor (BDNF). By dot blot the antibody shows less than 0.1% cross reactivity with recombinant human NT3, NT4/5 or mouse NGF.

Storage and Stability

Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Target description

18 kDa, with a homodimer at ~27-30 kDa