Application

Anti-phospho-SIN1 (Thr398), Cat. No. ABS1506, is a rabbit polyclonal antibody that detects Target of rapamycin complex 2 subunit MAPKAP1 (SIN1) and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Research CategorySignaling

Peptide Inhibition Analysis: A 1:500 dilution from a representative lot was used with L6 cells treated with IGF-1 for peptide block analysis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Target of rapamycin complex 2 subunit MAPKAP1 (UniProt: Q9BPZ7; also known as TORC2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, Stress-activated map kinase-interacting protein 1, SAPK-interacting protein 1, mSIN1) is encoded by the MAPKAP1 (also known as MIP1, SIN1) gene (Gene ID: 79109) in human. mSIN1 is a subunit of mTORC2 regulates cell growth and survival in response to hormonal signals. It is ubiquitously expressed, with highest levels in heart and skeletal muscle. Six isoforms of SIN1 have been described that are produced by alternative splicing. All isoforms with the exception of isoform 4 can be incorporated into the mTORC2 complex. mTORC2 is shown to be activated by growth factors, but in contrast to mTORC1, it is nutrient-insensitive. mTORC2 phosphorylates serine 473 in Akt1, which facilitates the phosphorylation of Threonine 308 by PDK1, thereby allowing full activation of Akt. Genetic knockout of SIN1 is reported to abolish serine 473 phosphorylation of Akt and disrupts the RICTOR-mTOR interaction. mTORC2 also regulates the phosphorylation of SGK1 at serine 422. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and auto-phosphorylation.

Immunogen

KLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphorylated Threonine 398 from human Stress-activated MAP kinase-interacting protein 1 (SIN1).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in L6 cells treated with IGF-1.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-SIN1 (Thr398) in lysate from L6 cells treated with IGF-1.

Specificity

This rabbit polyclonal antibody detects human Stress-activated MAP kinase-interacting protein 1 phosphorylated on Threonine 398.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~65 kDa observed; 59.12 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).