Application

Anti-β-Tubulin III antibody is suitable for use in flow cytometry (1:200-1:500), immunohistochemistry (1:100-1:200), immunoblot (approx. 50kDa) and western blot (1:500-1:1,000).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunocytochemistry (1 paper)Immunofluorescence (1 paper)Western Blotting (1 paper)

Biochem/physiol Actions

Missense mutations in the tubulin β 3 class III gene (TUBB3) impacts microtubule organisation in neuronal cells and contributes to neurological disorders. Mutations in TUBB3 is implicated in tubulopathies contributing to malformations in developing fetus.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Microtubules are heterodimeric proteins that comprise of α- and β-tubulins. β-tubulins have several different isotypes . β-Tubulin III is a marker for nerve cell differentiation, as well as for venous and lymphatic valves . Changes in β-tubulin III expression have been associated with medulloblastoma Anti-tubulin antibodies are useful to study the localization of tubulin proteins. Anti-β-Tubulin III antibody is specific for β-Tubulin III in humans.

Tubulin β 3 class III (TUBB3) is mapped to human chromosome 16q24.3.

Immunogen

synthetic peptide corresponding to residues 151-165 of human β-Tubulin III.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.