Analysis Note

Negative Control293T cells

Positive ControlHeLa cells

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)

General description

Purified mouse monoclonal antibody. Recognizes the ~52 kDa TNS4 protein.

Recognizes the ~52 kDa TNS4 protein in HeLa cells.

This Anti-TNS4 Mouse mAb (3B8) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of TNS4.

Immunogen

Full-length, recombinant human TNS4 (aa 1-481), expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Sakashita, K., et al. 2008. Ann Surg Oncol.15, 2606.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)