SAB3700679-1.5MG Display Image

Anti-Rat IgM (mu-chain specific)-Peroxidase antibody produced in rabbit, IgG fraction of antiserum, lyophilized powder

Code: SAB3700679-1.5MG D2-231

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to b...

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£234.00 EACH
List Price

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin M (IgM) has a high molecular weight and belongs to the immunoglobulin family. It exists as a pentamer in the serum. Monomeric IgM has a molecular weight of 190kDa. The pentameric IgM consists of ten µ-heavy chains, ten light chains and a single J-chain which are linked by disulphide bonds. The µ-chain consists of five domains- VH, Cµ1, Cµ2, Cµ3 and Cµ4. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as Fragment antigen binding (Fab) fragments. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The heavy chains of IgM contain an extra C domain that replaces the hinge region present between Fab and Fc. IgM is the first antibody to be produced during an immune response and is the major isotype secreted in T-cell independent immune responses.

Immunogen

Rat IgM mu heavy chain

Physical form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Physical properties

Antibody format: IgG

Reconstitution

Reconstitute with 1.0 mL deionized water (or equivalent).

Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Rat IgM and Rat Serum. No reaction was observed against Rat IgA or Rat IgG. ELISA was used to confirm specificity at less than 1% cross reactivity against other rat heavy or light chain isotypes.

application(s)western blot: suitable
application(s)indirect ELISA: suitable
application(s)immunohistochemistry: suitable
clonepolyclonal
antibody formaffinity isolated antibody
antibody product typesecondary antibodies
conjugateperoxidase conjugate
formlyophilized powder
Quality Level100
storage temp.2-8°C
shipped inwet ice
biological sourcerabbit
species reactivityrat