Application

Anti-NBS1 (Nibrin) antibody has been used:in immunofluorencein immunoblottingin immunoprecipitation

Biochem/physiol Actions

Nibrin (NBS1) is involved in stabilizing genomes and has a role in development of cancers. It also takes part in the repair mechanism after double strand breaks in the DNA.

NBS1 (Nibrin) was first isolated as a protein involved in DNA repair through analysis of mutations in patients with Nijmegen breakage syndrome (NBS). The majority of NBS patients bear a five base pairs deletion that leads to truncated NBS1, called 657del5. p95/NBS1 (nibrin) deficiency abrogates the formation of the MRE11/RAD50 ionizing radiation-induced foci, revealing a molecular link between DSB repair and cell cycle checkpoints activated by DNA damage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nibrin (NBS1) is made up of 754 amino acids.

NBS1 (Nibrin), also known as p95 protein of the meiotic recombination 11 homolog 1/DNA repair protein RAD50 (MRE11/RAD50) complex. NBS1 contains two domains found in the cell cycle checkpoint proteins forkhead-associated domain (FHA) and an adjacent breast cancer carboxyterminal domain (BRCT). NBS1 is one of the protein components of the double-strand break repair complex NBS1/MRE11/p50.

Immunogen

synthetic peptide corresponding to amino acids 692-706 of mouse NBS1 (nibrin), conjugated to KLH via an N-terminal added cysteine residue.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.