Positive ControlHeLa cells
ELISA (see comments)Immunoblotting (1-5 g/ml)
Recognizes the ~50 kDa IL13RA2 protein in HeLa cells.
Purified mouse monoclonal antibody. Recognizes the ~50 kDa IL13RA2 protein.
This Anti-IL13RA2 Mouse mAb (2E10) is validated for use in ELISA, Immunoblotting for the detection of IL13RA2.
A recombinant polypeptide corresponding to amino acids 27-127 of human IL13RA2, expressed as a GST fusion protein
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Zeis, T., et al. 2008. Brain131 288.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
In PBS, pH 7.2.
Following initial thaw, aliquot and freeze (-20°C).
Toxicity: Regulatory Review (Z)