Analysis Note

Positive ControlHeLa cells

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)

General description

Recognizes the ~50 kDa IL13RA2 protein in HeLa cells.

Purified mouse monoclonal antibody. Recognizes the ~50 kDa IL13RA2 protein.

This Anti-IL13RA2 Mouse mAb (2E10) is validated for use in ELISA, Immunoblotting for the detection of IL13RA2.

Immunogen

A recombinant polypeptide corresponding to amino acids 27-127 of human IL13RA2, expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Zeis, T., et al. 2008. Brain131 288.

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)