Analysis Note

ControlPositive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.

Application

Anti-Actin Antibody, smooth muscle, clone ASM-1 detects level of Actin & has been published & validated for use in IF, IH, IH(P) & WB.

Immunohistochemistry:
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.

Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.

Optimal working dilutions must be determined by the end user.

Research CategoryCell Structure

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. This antibody reacts with many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues, and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Myogenic soft tissues detected include leiomyosarcomas, leiomyomas, and certain stromal cells surrounding infiltrating ductal carcinoma of the breast.

Immunogen

Epitope: N-terminus

Synthetic peptide corresponding to the ten N-terminal amino acids of the alpha-smooth muscle isoform of actin.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1040

Physical form

Protein A purified

Purified mouse monoclonal IgG2a presented as lyophilized material. Reconstitute in 1 mL of of distilled water.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot on HUVEC lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 µg of HUVEC lysates.

Specificity

This antibody is specific for the alpha-smooth-muscle isoform of actin (MW 43 kDa).

Expected to cross-react with bovine, equine and chicken. Reactivity with other species has not been confirmed.

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

43 kDa